在线高分求英语大神帮忙翻译(英译汉)~医学方向，专业性较强，不求完美但求能读的通……翻译器勿扰谢谢~

Question

After separation, the head and testes ofmales were removed and purifiedtotal RNA was prepared from the remaining tissue from pools of 14–18parasites using Trizol (Invitrogen) andDNase treatment (DNA-free RNAkit, ZymoResearch).Three independent biological replicateswereperformed for both control and irradiated experimental groups. Individuallytagged libraries forRNA-seqwere prepared (TruSeqRNaseq Sample prep kit, Illumina), pooled in a singlelane, and 100-bp reads were generated using an Illumina HiSeq2000. Library preparationand Illumina sequencing were performed at theW.M. Keck Center for Comparativeand Functional Genomics. The resulting reads were mapped to the annotated S.mansoni genome6 (v5.0) and differences in gene expression were determined using CLCGenomics Workbench (CLC bio). Statistical enrichment of Gene Ontology terms wasdetermined in CLC Genomics Workbench using a hyper geometric test that issimilar to theGOstat test described in previous studies36.To examinesimilarities between proteins encoded fromirradiation-sensitive transcripts in S.mansoni and genes expressed in planarian neoblasts, we compared our schistosomedata setwith both neoblast-enriched and ‘whole’transcriptomes14,15,18,19 using standalone tBLASTn. Schistosome proteins sharing no similarity totranslated planarian mRNAs (e-value cutoff .131025) were omitted fromanalysis.

这些是翻译中的一部分，还有一小部分……求发邮箱[email protected]

Answer 1

After separation, the head and testes of males were removed and purifiedtotal RNA was prepared from the remaining tissue (组织) from pools () of 14–18 parasites(寄生虫) usingTrizol (Invitrogen) and DNase treatment (DNA-free RNA kit Zymo Research).Threeindependent biological replicates were performed for both control andirradiated (辐射/扩散) experimental groups.
　　男性阴囊前端和睾丸分离以后，它们被取下来，已清洁过的全部核糖核酸从14—18寄生物淤积产生的保留组织中用蒂罗尔（Invitrogen生命技术公司）和脱氧核糖核酸酶处理（核糖核酸分离的脱氧核糖核酸工具包酶解研究）法配制。对控制实验组和扩展实验组两方面来说，三种单独的生物复制被完成。
Individually tagged libraries for RNA-seq were prepared (TruSeq RNaseqSample prep kit, Illumina), pooled in a single lane, and 100-bp reads weregenerated using an Illumina HiSeq2000. Library preparation and Illuminasequencing were performed at the W.M. Keck Centerfor Comparative and Functional Genomics.
　　准备了为核糖核酸遗留物单独标记的程序库（完成的遗留物/核糖核酸遗留物样本准备工具包腔管），汇集在一个单通道中，并用一个高密度遗留物2000腔管生成100气压。在W.M. Keck中心对“比较和功能染色体组”完成了程序库准备和腔管排序。
The resulting reads were mapped to the annotated S. mansoni genome 6(v5.0) and differences in gene expression were determined using CLC GenomicsWorkbench (CLC bio). Statistical enrichment of Gene Ontology terms was determinedin CLC Genomics Workbench using a hyper geometric test that is similar to the GOstattest described in previous studies 36.
　　把其结果的读数绘制到有标注的 S. 曼森尼（mansoni）基因组6 (v5.0) 中，基因表达中的区别用CLC染色体组工作平台（CLC生物）被确定下来。用与前面研究36中表述的运行状态测试类似的超几何分布测试，基因本体术语的统计集成在CLC染色体组工作平台中被确定下来。
To examine similarities between proteins encoded from irradiation-sensitivetranscripts in S. mansoni and genes expressed in planarian neoblasts, wecompared our Schistosome data set with both neoblast-enriched and ‘whole’ transcriptomes14,15,18,19using standalone tBLASTn. Schistosome proteins sharing no similarity totranslated planarian mRNAs (e-value cutoff .131025) were omitted from analysis.
　　为了在S. 曼森尼中由辐射敏感的转录物编码的蛋白质与涡虫未分化细胞中表现出来的遗传基因之间检查相似性，我们用独立的tBLASTn对我们的血吸虫数据组与未分化细胞聚集和“全部的”转录组14,15,18,19双方面进行比对。血吸虫蛋白质与转变为涡虫的mRNAs (e-value cutoff .131025)同时不具有相似性这一点在分析中被忽略不计。

注：太专业，只能翻译到此种程度，仅供参考。追问

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打开链接显示的是 “知道宝贝找不到问题了>_<!! ，该问题可能已经失效”

Answer 2

分离之后，雄性的head和testes被移除，净化的总RNA从剩下的14-18寄生组织里面用Trizol和DNase treatment提取出来（或说：总RNA从剩下的14-18寄生组织里面用Trizol和DNase treatment净化分离出来）。三个独立的生物学对控制的（controlled）和实验照射（irradiated experimental)两组进行复制。失去耐心了。哈哈哈。我再继续写点，看着还行就给点分，那我就比较有动力了。前句有点不通。重新写下。独立对控制的（controlled）和实验照射（irradiated experimental)两组进行三个独立的生物学复制。RNA-seq(大概是RNA-Sequence,RNA排列？）用True RNA-seq Sample pre Kit, Illumina逐个标记成样板（样板来翻library有点那个，专业人士一定有更好的词），收集在singlelane, 并且用ILLUMINA HiSeq2000来生成100-bp的数据。样板标记和Illumina排列是在w.m. keck center for comparative and functional genomics进行的。结果数据与notated(加注的？）s.mansoni genome6(v5.0)进行比照，基因表达差异的地方通过clc genomics workbench（这是一个软件吗？）决定。基因本体的统计富集（gene ontology=基因本体；statistical enrichment=统计富集，这两个词都是google translate翻译的）是通过clc genomics workbench用类似于前一研究go stat test 的hyper geometric test方法决定的。 为了检验proteins encoded from irradiation-senstive transcripts in s.mansoni和genes experssed in planarian neoblasts的相似处，我们对我们的schist与有neoblast-enriche+ whole transcript omes14151819的一些数据通过独立的tBlastn进行了比较。 schist 和（？）一些与translated planarian mRNAs(e-value cutoff .131025)没有相似度的蛋白质忽略不计。（我对翻译论文什么的很有兴趣，有需要可以找我。）

Answer 3

分离后，男性被拆除，从剩余的组织从14–18parasites采用Trizol池备purifiedtotal RNA头部和睾丸（Invitrogen公司）anddnase治疗（DNA自由rnakit，zymoresearch）。三个独立的生物replicateswereperformed为对照组和照射组。individuallytagged图书馆forrna seqwere准备（truseqrnaseq样品制备试剂盒，Illumina公司），汇集在一个singlelane，和100 bp的读取使用Illumina公司的hiseq2000产生。在该图书馆和Illumina的测序。对于比较功能基因组学中心的凯克。由此产生的读取映射到注释的曼氏血吸虫genome6（5.0）和基因表达的差异，以确定clcgenomics工作台（CLC生物）。基因本体术语的结合使用超几何测试，你thegostat试验前studies36描述在CLC基因组学平台统计富集。examinesimilarities之间对编码的蛋白转录fromirradiation敏感的曼氏血吸虫和基因在涡虫neoblasts表示，我们比较schistosomedata存在两neoblast丰富和“whole'transcriptomes14,15,18,19使用独立的获得。血吸虫蛋白共享不相似totranslated涡虫基因（E值截止。131025）被省略分析。