Objective to explore the Rosiglitazone (Rosiglitazone) for ovarian cancer cell lines SKOV3 cell apoptosis function, and its cell apoptosis and telomerase reverse transcriptase (human telomerase reverse transcriptase, hTERT) the relation between. Methods using the cart before the horse differ microscope before and after drug cells form change; Drug 24 and 48 hours after the flow cytometric respectively SKOV3 detecting oarian cancer cell apoptosis situation; RT-PCR detection hTERT genetic change; Immune fluorescence detection rosiglitazone role hTERT expression after changes. The results of rosiglitazone treatment, the cart before the horse differ microscope detected ovarian cancer cell to go round, cytoplasm shrinks, intercellular broadens, some cells to take off the wall and floating. 25 ~ 200 μ mol/L rosiglitazone role in cells after 24 h happens namely cell apoptosis, 48 h apoptosis more apparent, at the same time, time and concentration dependence exists. 48 h role after RT-PCR detection hTERT mRNA expression of the cut, the immune hTERT fluorescent detecting protein expression cut, in concentration dependence. Conclusions rosiglitazone can induce apoptosis SKOV3 ovarian cancer cell lines, with time concentration dependence, its mechanism and the expression of the hTERT may cut about.
追问好像不太行啊，嘿嘿，不管怎样，谢谢你。