参考： 南京农业大学微生物考研面试试题 What’s the koch’s postulate? Would you please describe the roles of microorganisms in nitrogen and sulfur cycle? Would you please describe the roles of microorganisms in the environmental protection? How many methods are used to bio treat waste water? How could E.coli degrade glucose completely into CO2 and H2O? Explain how the Gram stain works to distinguish between the Gram-positive and Gram-negative cell wall? Give the reason that chemoautotrophic bacteria grow very slowly? What are the characteristic features of bacterial respiration compared with that of eukaryotes? How to use bacterial growth curve to instruct your researchers or industry fermentation? Describe the main steps of gene engineering? What’s the difference of fermentation and respiration? How many groups of microorganisms are classified according to growth temperature/ How do you think about the diversities of microorganism metabolism? How many methods are there to measure the microorganism growth? Gram-negative bacteria are generally more resistant to disinfectants and antiseptics than Gram-positive bacteria. Why? How many types of horizontal gene transfer are there in bacteria? Give the examples of application of DNA recombination in agriculture. What’s the southern blot? What’s the northern blot? What’s the western blot? What’s the shuttle vector? Why are endospores highly resistant to heat? What are the advantages of bioremediation compared with the chemical and physical remediation of polluted soil? How many groups are microorganisms classified according to their carbon and energy source? How do you think about diversities of microorganisms? Describe the roles of microorganisms in agriculture. Describe the process of compost pile and roles microorganisms take. How to detect the bacteria inoculated into soil?