帮帮忙，帮我把已经弄的好的基因序列用什么什么生物软件分析一下，我研究的是螺原体。

Question

>spiralin
ATGAAAAAATTATTAGCAATCTTAGGAGTAATTAGTCTTGTAACAACAAGTTCTATCAATGTTGTTAGTTGTCATCAAAAAGAAAGCAGTAATAAAATTGATTTAAAAACAATTATTAAAACGACGGATTTGGGAAAAATACCCAATGAAGACATTAATAATATTATTAATCTGCTTTGTGCAAAAAATCCATATTTAGATGCTACACAAATTAAGATACAGCATCTTGCGGAAAATTCTGCGGTAATTTTAGCCAACGATAATTCTTGAAAATATTTCGGGAAAGTTAATATTACTTTTGAAGTTGCCCATGCTGTTGATATTACTAGTGTAACATTGCCCCAAGAAACCATAAAAATTTTAGTATCGAACCAACAAAAGGTTAGTGCTGGTGAGTTAAACAAAATAAATACCGAACCAATCTTATTGCAAGATATTATTAAAGCAATTGATACTCAATTAGAAGTTAATGTTACTAACAATGATTTTATAGTTACTAATAATGCTCAAAGTGGAGATTATAGTAACCCCCAAACAATTGAAATTACAGTAACCAGTGCAACAACTTCATTTCTTATTAAGGGAGCATTTATTTTTACCGCACAGTTATCAACAACTACTCAACAAACTGATATTAGCGGGATTACTAAATTAAAAACCCCGTCTTATTTTGGTGTAAGTAATCCATCGGAAGTCTTTGCTGAAGATATTCGCCAACTATTGGATCCGATTATCACCGCTGCTGTGCAAAATATTAAAAGCACGCTAACAAGTCGTGATTTTACTTATACCTTAACAGT
TAAATGAGATGGGCGTTATTATTGACCGGGTCATCATTGATGAGACCAATATCTTGATTTGTCTTCCAAAAGTAAAACTATCCAAGTTCAAATTAAAGGAACCGGTAATGCTACTGGAGAGACACCTAATATTAATGTGACCTTACCAAAAGCAGTTAAGAAATAA

Answer 1

要分析成什么样子啊？
Molecular Weight 34525.35 Daltons
316 Amino Acids
29 Strongly Basic(+) Amino Acids (K,R)
29 Strongly Acidic(-) Amino Acids (D,E)
113 Hydrophobic Amino Acids (A,I,L,F,W,V)
115 Polar Amino Acids (N,C,Q,S,T,Y)

7.374 Isolectric Point
0.688 Charge at PH 7.0

Total number of bases translated is 966
% A = 37.78 [365]
% G = 15.63 [151]
% T = 30.54 [295]
% C = 16.05 [155]
% Ambiguous = 0.00 [0]

% A+T = 68.32 [660]
% C+G = 31.68 [306]

BASE COUNT 365 a 155 c 151 g 295 t

Davis,Botstein,Roth Melting Temp C. 77.37
Wallace Temp C 2544.00
Codon usage:
gca Ala(A) 7 # cag Gln(Q) 2 # uug Leu(L) 5 # uaa Ter(.) 1
gcc Ala(A) 2 # --- Gln(Q) 18 # --- Leu(L) 27 # uag Ter(.) 0
gcg Ala(A) 2 # gaa Glu(E) 10 # aaa Lys(K) 22 # uga Ter(.) 5
gcu Ala(A) 8 # gag Glu(E) 2 # aag Lys(K) 4 # --- Ter(.) 6
--- Ala(A) 19 # --- Glu(E) 12 # --- Lys(K) 26 # aca Thr(T) 12
aga Arg(R) 0 # gga Gly(G) 6 # aug Met(M) 1 # acc Thr(T) 9
agg Arg(R) 0 # ggc Gly(G) 0 # --- Met(M) 1 # acg Thr(T) 3
cga Arg(R) 0 # ggg Gly(G) 3 # uuc Phe(F) 1 # acu Thr(T) 13
cgc Arg(R) 1 # ggu Gly(G) 4 # uuu Phe(F) 8 # --- Thr(T) 37
cgg Arg(R) 0 # --- Gly(G) 13 # --- Phe(F) 9 # ugg Trp(W) 0
cgu Arg(R) 2 # cac His(H) 0 # cca Pro(P) 4 # --- Trp(W) 0
--- Arg(R) 3 # cau His(H) 5 # ccc Pro(P) 3 # uac Tyr(Y) 0
aac Asn(N) 5 # --- His(H) 5 # ccg Pro(P) 3 # uau Tyr(Y) 8
aau Asn(N) 20 # aua Ile(I) 5 # ccu Pro(P) 1 # --- Tyr(Y) 8
--- Asn(N) 25 # auc Ile(I) 5 # --- Pro(P) 11 # gua Val(V) 7
gac Asp(D) 2 # auu Ile(I) 26 # agc Ser(S) 3 # guc Val(V) 1
gau Asp(D) 15 # --- Ile(I) 36 # agu Ser(S) 12 # gug Val(V) 2
--- Asp(D) 17 # cua Leu(L) 2 # uca Ser(S) 2 # guu Val(V) 12
ugc Cys(C) 0 # cuc Leu(L) 0 # ucc Ser(S) 1 # --- Val(V) 22
ugu Cys(C) 2 # cug Leu(L) 1 # ucg Ser(S) 2 # nnn ???(X) 0
--- Cys(C) 2 # cuu Leu(L) 5 # ucu Ser(S) 5 # TOTAL 322
caa Gln(Q) 16 # uua Leu(L) 14 # --- Ser(S) 25 #
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Created: Wednesday, May 18, 2011 05:12 PM

Answer 2

推荐用bowtie，也可以上NCBI去检索一下，用blast或者genbank。自己弄弄就会用了，其余的无法代劳了。