Studies have indicated that the medium composition and
growth conditions can influence the type and yield of biosur-
factant. Carbon source influences biosurfactant synthesis by
either induction or repression. In some cases addition of
water-immiscible substrates results in induction of biosurfac-
tant production. The induction of sophorolipid synthesis by
addition of long-chain fatty acids, hydrocarbons, or glycerides
to growth media of Torulopsis magnoliae (14), of trehalolipid
synthesis in Rhodococcus erythropolis by addition of hydrocar-bons (15), and of glycolipid-EM (where EM = emulsifier) in
P. aeruginosa SB-30 by addition of alkanes (16) has been re-
ported. Induction also regulates synthesis of many lipopep-
tide biosurfactants (17–19). Banat et al. (20) observed little
biosurfactant production when cells were growing on a read-
ily available carbon source. Only when the soluble carbon was
fully consumed and a water-immiscible hydrocarbon was
available was biosurfactant production triggered. A carbon
source in the medium, particularly a carbohydrate, has con-
siderable bearing on the type of glycolipids formed. Suzuki et
al. (21) observed that glucose, fructose, and sucrose lipids are
formed by Arthrobacter paraffineus and several species of
Corynebacterium, Nocardia, and Brevibacterium during growth
on the corresponding sugar. The Bacillus strain used in this
study is able to utilize glucose, sucrose, and sodium pyruvate
for biosurfactant production. The biosurfactant was not pro-
duced when sodium acetate was used as the carbon source.
The strain was able to utilize n-hexadecane and pristane for
growth but not for biosurfactant production. Sucrose was
found to be a suitable carbon source for maximal growth and
biosurfactant production and was studied at a concentration
of 2% in minimal medium.There is evidence that nitrogen plays an important (defi-
nite) role in the production of surface-active compounds by
microorganisms. Arthrobacter paraffineus ATCC 19558 pre-
ferred ammonium to nitrate for biosurfactant production.
Urea also resulted in good surfactant production (22). An in-
vestigation of rhamnolipid production by Pseudomonas 44Ti
on olive oil showed that sodium nitrate was the best nitrogen
source (23). Syldatk et al. (24) showed that nitrogen limita-
tion increased the production of some biosurfactants but
also changed the composition of the biosurfactants. In the
present study, both sodium nitrate and potassium nitrate are
preferred nitrogen sources. Bacillus subtilis MTCC 2423 was
not able to utilize ammonium sulfate, but exhibited a prefer-
ence for nitrate ions. Potassium nitrate (3g/L) was found to
be optimal for biosurfactant production. The biosurfactant
production was ca.1.0 g/L with urea.
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