参考资料：
https://www.atcc.org/products/all/CRL-2254.aspx#generalinformation
This cell line is a suitable transfection host.
该细胞系适宜转染。
The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha.
AML12(alpha小鼠肝12)细胞系来源于(CD1，MT42品系)人源TGF -alpha转基因小鼠的的肝实质细胞。
albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha.
白蛋白;人源转化生长因子(TGF);鼠源转化生长因子α。
albumin; human transforming growth factor alpha (TGF alpha); mouse TGF alpha.
白蛋白;人源转化生长因子(TGFα);鼠源TGFα。
No, the cells do not form colonies or grow in soft agar.
这些细胞不会形成克隆，也不会在软琼脂中生长。
No, the cells were not tumorigenic in immunosuppressed mice.
这些细胞在免疫抑制小鼠中不具有致瘤性。
The AML12 (alpha mouse liver 12) cell line was established from hepatocytes from a mouse (CD1 strain, line MT42) transgenic for human TGF alpha.
AML12(alpha小鼠肝12)细胞系来源于(CD1，MT42品系)人源TGF -alpha转基因小鼠的的肝实质细胞。
By electron microscopy, these cells exhibit typical hepatocyte features such as peroxisomes and bile canalicular like structure.
通过电镜观察，这些细胞表现出典型的肝细胞特征，如 过氧化物酶体 和胆管样结构。
AML12 cells retain the capacity to express high levels of mRNA for serum (albumin, alpha 1 antitrypsin and transferrin) and gap junction (connexins 26 and 32) proteins, and contain solely isoenzyme 5 of lactate dehydrogenase.
AML12细胞保留了高水平(血清白蛋白、α1抗胰蛋白酶和转铁蛋白)和缝隙连接(连接蛋白26和32)蛋白mRNA的能力，并且仅含有乳酸脱氢酶同工酶5。
The cells express high levels of human TGF alpha and lower levels of mouse TGF alpha.
细胞表达高水平的人TGFα，低水平的小鼠TGFα。
Expression of liver specific proteins decreases with time in culture, but is reactivated by growing the cells in serum free medium.
肝脏特异性蛋白的表达随培养时间的延长而降低，但在无血清培养基中通过细胞生长而重新激活。
The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following component to the 500 mL of the base medium:
该细胞系的基础培养基是DMEM：F12。要制作完全培养基，请将以下成分添加到500 mL的基础培养基中：
This medium is formulated for use with a 5% CO2 in air atmosphere.
该培养基可在空气中通入5％CO 2使用。
Subculturing传代
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
该方案中使用的容积为75 cm2培养瓶；其他尺寸培养皿，按比例减少或增加培养基量。
Subcultivation Ratio: 1:4 to 1:6
传代比例：1:4 - 1:6
Medium Renewal: 2 to 3 times a week.
培养基更换频率：一周2 - 3次
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
冻存培养基：完全生长培养基，添加5％（v / v）DMSO
Storage temperature: liquid nitrogen vapor phase
储存温度：液氮
Temperature: 37°C